The U.S. AI investment landscape in 2025 continues its explosive trajectory, building on the momentum of a record-breaking 2024, when 49 startups raised $100 million or more. As of mid-2025, at least 25 AI startups have already crossed that threshold, signaling sustained investor confidence and accelerating sector growth. While foundational models and general AI applications led the charge—with giants like OpenAI, Anthropic, and xAI securing a staggering $53.5 billion collectively—AI infrastructure is emerging as a critical area for funding. Startups such as Lambda, Together AI, and Celestial AI have raised hundreds of millions to strengthen computing, data, and chip ecosystems. Other booming sectors include AI programming tools, enterprise search, quantum AI, and generative AI video platforms like Runway.
Healthcare is one of the most prominent verticals benefitting from AI investment, with Hippocratic AI standing out as a key player. In January 2025, the company secured $141 million in a Series B round, bringing its total funding to $278 million and valuing the startup at over $1.6 billion. Backed by Kleiner Perkins, Andreessen Horowitz, Nvidia, and General Catalyst, Hippocratic AI is developing large language models specifically tailored for healthcare, supporting scalable, non-diagnostic applications with an emphasis on safety and empathy. Its success reflects a broader investor shift toward AI companies with real-world utility and commercial viability—especially in regulated industries like medicine and law. Alongside peers such as Abridge and Harvey, Hippocratic AI exemplifies how targeted, sector-specific AI is becoming a core pillar of the next generation of technology investment.